McMaster University
Next Generation Sequencing Print E-mail

Mobixlab is now offering Next Generation Sequencing (NGS) services through Farncombe  Metagenomics  Facility.  Presently, our NGS service is available for the 454-GS FLX (Roche).  The 454-GS FLX system features long reads (up to 400 bases), exceptional accuracy and high-throughput.  It is well suited for large genomic projects.

Available capabilities include:

  •  De novo or re-sequencing of whole or partial genomes
  •  RNA-Seq (cDNA libraries)
  •  Target re-sequencing
  •  Amplicon Sequencing

The target DNA is required to be processed into a DNA library (flanked by specific adapters).  Libraries can be processed by us (for a fee) or by the customer, using the specific kit and manufacturer’s protocol. 

Using the 454-GS FLX, adapters with barcodes (MIDs) are available which enable multiple libraries to be run simultaneously on the same plate/run. 

The expected number of reads varies with the quality of the library and the plate configuration, but on average you can expect:

Plate

Region Size

Regions per Plate

Bases per Region (Mbp)

Bases per Full plate (Mbp)

Reads per Region

(x 103)

Large

2

180-280

360-560

450-650

Medium

4

60-110

240-440

160-250

M/S

8

30-55

240-440

80-120

Small

16

10-20

160-320

25-40

 

Please contact This e-mail address is being protected from spambots. You need JavaScript enabled to view it , (905)525-9140, ext. 27048
or This e-mail address is being protected from spambots. You need JavaScript enabled to view it at Farncombe Metagenomics Facility (905)525-9140, ext. 28013
about pricing and advice regarding your specific project needs.